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Does centrifugation matter? Centrifugal force and spinning time alter the plasma metabolome.

ABSTRACT:

Background

Centrifugation is an indispensable procedure for plasma sample preparation, but applied conditions can vary between labs.

Aim

Determine whether routinely used plasma centrifugation protocols (1500×g 10 min; 3000×g 5 min) influence non-targeted metabolomic analyses.

Methods

Nuclear magnetic resonance spectroscopy (NMR) and High Resolution Mass Spectrometry (HRMS) data were evaluated with sparse partial least squares discriminant analyses and compared with cell count measurements.

Results

Besides significant differences in platelet count, we identified substantial alterations in NMR and HRMS data related to the different centrifugation protocols.

Conclusion

Already minor differences in plasma centrifugation can significantly influence metabolomic patterns and potentially bias metabolomics studies.

SUBMITTER: Lesche D

PROVIDER: S-EPMC5025507 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Publications

Does centrifugation matter? Centrifugal force and spinning time alter the plasma metabolome.

Lesche Dorothea D Geyer Roland R Lienhard Daniel D Nakas Christos T CT Diserens Gaëlle G Vermathen Peter P Leichtle Alexander B AB

Metabolomics : Official journal of the Metabolomic Society 20160915 10

<h4>Background</h4>Centrifugation is an indispensable procedure for plasma sample preparation, but applied conditions can vary between labs.<h4>Aim</h4>Determine whether routinely used plasma centrifugation protocols (1500×<i>g</i> 10 min; 3000×<i>g</i> 5 min) influence non-targeted metabolomic analyses.<h4>Methods</h4>Nuclear magnetic resonance spectroscopy (NMR) and High Resolution Mass Spectrometry (HRMS) data were evaluated with sparse partial least squares discriminant analyses and compared ...[more]

PMID: 27729833

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