GEMINI: Initial behavioral results after full severance of the cervical spinal cord in mice.
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ABSTRACT: Background
The GEMINI spinal cord fusion protocol has been developed to achieve a successful cephalosomatic anastomosis. Here, we report the preliminary data on the use of a fusogen [polyethylene glycol (PEG)] after full cervical cord transection in mice to facilitate the fusion of both ends of a sharply transected spinal cord.Methods
Cervical laminectomy and a complete, visually confirmed cervical cord (C 5) transection was performed on female albino mice (n = 16). In Group 1 (n = 8), a fusogen, (PEG) was used to bridge the gap between the cut ends of the spinal cord. Group 2 received the same spinal cord transection but was treated with saline. Outcome was assessed daily using a standard scale (modified 22-point Basso-Beattie-Bresnahan scale) and filmed on camera.Results
The PEG group (group 1) showed partial restoration of motor function after 4 weeks of observation; group 2 (placebo) did not recover any useful motor activity.Conclusion
In this preliminary experiment, PEG, but not saline, promoted partial motor recovery in mice submitted to full cervical transection.
SUBMITTER: Kim CY
PROVIDER: S-EPMC5025949 | biostudies-literature |
REPOSITORIES: biostudies-literature
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