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The effect of microbial colonization on the host proteome varies by gastrointestinal location.

ABSTRACT: Endogenous intestinal microbiota have wide-ranging and largely uncharacterized effects on host physiology. Here, we used reverse-phase liquid chromatography-coupled tandem mass spectrometry to define the mouse intestinal proteome in the stomach, jejunum, ileum, cecum and proximal colon under three colonization states: germ-free (GF), monocolonized with Bacteroides thetaiotaomicron and conventionally raised (CR). Our analysis revealed distinct proteomic abundance profiles along the gastrointestinal (GI) tract. Unsupervised clustering showed that host protein abundance primarily depended on GI location rather than colonization state and specific proteins and functions that defined these locations were identified by random forest classifications. K-means clustering of protein abundance across locations revealed substantial differences in host protein production between CR mice relative to GF and monocolonized mice. Finally, comparison with fecal proteomic data sets suggested that the identities of stool proteins are not biased to any region of the GI tract, but are substantially impacted by the microbiota in the distal colon.

SUBMITTER: Lichtman JS

PROVIDER: S-EPMC5029216 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature

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The effect of microbial colonization on the host proteome varies by gastrointestinal location.

Lichtman Joshua S JS Alsentzer Emily E Jaffe Mia M Sprockett Daniel D Masutani Evan E Ikwa Elvis E Fragiadakis Gabriela K GK Clifford David D Huang Bevan Emma BE Sonnenburg Justin L JL Huang Kerwyn Casey KC Elias Joshua E JE

The ISME journal 20151117 5

Endogenous intestinal microbiota have wide-ranging and largely uncharacterized effects on host physiology. Here, we used reverse-phase liquid chromatography-coupled tandem mass spectrometry to define the mouse intestinal proteome in the stomach, jejunum, ileum, cecum and proximal colon under three colonization states: germ-free (GF), monocolonized with Bacteroides thetaiotaomicron and conventionally raised (CR). Our analysis revealed distinct proteomic abundance profiles along the gastrointestin ...[more]

PMID: 26574685

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The effect of microbial colonization on the host proteome varies by gastrointestinal location.

Publications

The effect of microbial colonization on the host proteome varies by gastrointestinal location.

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