Unknown

Dataset Information

0

A Screening Method for the Isolation of Polyhydroxyalkanoate-Producing Purple Non-sulfur Photosynthetic Bacteria from Natural Seawater.


ABSTRACT: Polyhydroxyalkanoates (PHAs) are a family of biopolyesters accumulated by a variety of microorganisms as carbon and energy storage under starvation conditions. We focused on marine purple non-sulfur photosynthetic bacteria as host microorganisms for PHA production and developed a method for their isolation from natural seawater. To identify novel PHA-producing marine purple non-sulfur photosynthetic bacteria, natural seawaters were cultured in nutrient-rich medium for purple non-sulfur photosynthetic bacteria, and twelve pink- or red-pigmented colonies were picked up. Gas chromatography mass spectrometry analysis revealed that four isolates synthesized PHA at levels ranging from 0.5 to 24.4 wt% of cell dry weight. The 16S ribosomal RNA sequence analysis revealed that one isolate (HM2) showed 100% identity to marine purple non-sulfur photosynthetic bacteria. In conclusion, we have demonstrated in this study that PHA-producing marine purple non-sulfur photosynthetic bacteria can be isolated from natural seawater under nutrient-rich conditions.

SUBMITTER: Higuchi-Takeuchi M 

PROVIDER: S-EPMC5030216 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

altmetric image

Publications

A Screening Method for the Isolation of Polyhydroxyalkanoate-Producing Purple Non-sulfur Photosynthetic Bacteria from Natural Seawater.

Higuchi-Takeuchi Mieko M   Morisaki Kumiko K   Numata Keiji K  

Frontiers in microbiology 20160921


Polyhydroxyalkanoates (PHAs) are a family of biopolyesters accumulated by a variety of microorganisms as carbon and energy storage under starvation conditions. We focused on marine purple non-sulfur photosynthetic bacteria as host microorganisms for PHA production and developed a method for their isolation from natural seawater. To identify novel PHA-producing marine purple non-sulfur photosynthetic bacteria, natural seawaters were cultured in nutrient-rich medium for purple non-sulfur photosynt  ...[more]

Similar Datasets

| S-EPMC8130110 | biostudies-literature
| S-EPMC8307300 | biostudies-literature
| S-EPMC6546801 | biostudies-literature
| S-EPMC10686062 | biostudies-literature
| S-EPMC7118184 | biostudies-literature
| S-EPMC5125414 | biostudies-literature
| S-EPMC7697693 | biostudies-literature
| S-EPMC161547 | biostudies-literature
| S-EPMC6406701 | biostudies-literature
| S-EPMC2238716 | biostudies-literature