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Cytokines in saliva increase in head and neck cancer patients after treatment.

ABSTRACT: Approximately one-third of advanced head and neck squamous cell carcinomas (HNSCCs) recur within 2 years of treatment. Due to ease of collection, saliva is of interest to monitor changes that correlate with treatment. Previously this was a challenge due to xerostomia after conventional radiation. The emergence of gland-sparing radiation has made it possible to collect saliva post-treatment. This study investigated changes in cytokines in saliva pre- and post-treatment to provide foundational knowledge for future studies exploring the use of saliva to monitor treatment response.Pre- and post-treatment saliva was evaluated for 8 cytokines by multiplex assay and enzyme-linked immunosorbent assay.In oropharyngeal HNSCC, secretion of epidermal growth factor (EGF), GRO? (Growth-regulated protein alpha), interleukin (IL)-1?, IL-?, IL-6, IL-8, tumor necrosis factor-?, and vascular endothelial growth factor increased significantly post-treatment. In additional patients, significant increases of GR-? and IL-6 were validated, but EGF showed no change.The uniqueness of this study is its comparison of salivary cytokines from HNSCC patients pre- and post-treatment.

SUBMITTER: Russo N

PROVIDER: S-EPMC5031552 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature

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Cytokines in saliva increase in head and neck cancer patients after treatment.

Russo Nickole N Bellile Emily E Murdoch-Kinch Carol Anne CA Liu Min M Eisbruch Avi A Wolf Greg T GT D'Silva Nisha J NJ

Oral surgery, oral medicine, oral pathology and oral radiology 20160609 4

<h4>Objective</h4>Approximately one-third of advanced head and neck squamous cell carcinomas (HNSCCs) recur within 2 years of treatment. Due to ease of collection, saliva is of interest to monitor changes that correlate with treatment. Previously this was a challenge due to xerostomia after conventional radiation. The emergence of gland-sparing radiation has made it possible to collect saliva post-treatment. This study investigated changes in cytokines in saliva pre- and post-treatment to provid ...[more]

PMID: 27554375

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Project description:BackgroundTo analyse over time changes in stimulated whole saliva regarding total protein, Immunoglobulin A (IgA), and mucin type O-glycans (mostly MUC5B and MUC7) in head and neck cancer patients.Methods29 dentate patients (20 men and 9 women, 59 ± 8 years) treated with curative radiation therapy and chemotherapy for cancer of the head and neck region were included. The stimulated whole salivary secretion rate was determined and saliva collected at four time-points: at pretreatment, and at 6 months, 1 and 2 years post treatment. The total protein concentration was determined spectrophotometrically by using Bicinchoninic Acid assay and Immunoglobulin A (IgA) by using ELISA technique. Glycosylation pattern of salivary mucins was determined in samples collected pre- and post treatment by using LC/MS electrospray and mucin content quantified using SDS-AgPAGE gels and PAS staining.ResultsCompared with pretreatment, the total protein concentration was increased already at 6 months post treatment (p < 0.01), and continued to increase up to 2 years post treatment (p < 0.001). During that period no significant changes in IgA concentration was detected. At pretreatment, the output/min of both total protein and IgA was significantly higher than at all time-points post treatment. Saliva from the cancer patients showed a low abundance/no detectable MUC7, while the MUC5B level remained, compared to saliva from a healthy control. The glycomic analysis showed that the percentage of core 2 O-glycans was increased as core 1, 3 and 4 O-glycans were decreased. The level of sialylation was higher at 6 months post treatment, while sulfation was lower.ConclusionA decreased output per minute of proteins at decreased salivary secretion rate, as well as reduced sulfation of MUC5B at 6 months post treatment tended to correlate with the patients' experience of sticky saliva and oral dryness. At 2 years post treatment, the decreased amount of IgA combined with a lowered salivary secretion rate indicate a reduced oral defense with increased risk of oral infections.

Dataset Information

Cytokines in saliva increase in head and neck cancer patients after treatment.

Publications

Cytokines in saliva increase in head and neck cancer patients after treatment.

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