Ontology highlight
ABSTRACT:
SUBMITTER: Liu Z
PROVIDER: S-EPMC5032288 | biostudies-literature | 2016 Sep
REPOSITORIES: biostudies-literature
Liu Zhongliang Z Hui Yi Y Shi Lei L Chen Zhenyu Z Xu Xiangjie X Chi Liankai L Fan Beibei B Fang Yujiang Y Liu Yang Y Ma Lin L Wang Yiran Y Xiao Lei L Zhang Quanbin Q Jin Guohua G Liu Ling L Zhang Xiaoqing X
Stem cell reports 20160901 3
Loss-of-function studies in human pluripotent stem cells (hPSCs) require efficient methodologies for lesion of genes of interest. Here, we introduce a donor-free paired gRNA-guided CRISPR/Cas9 knockout strategy (paired-KO) for efficient and rapid gene ablation in hPSCs. Through paired-KO, we succeeded in targeting all genes of interest with high biallelic targeting efficiencies. More importantly, during paired-KO, the cleaved DNA was repaired mostly through direct end joining without insertions/ ...[more]