Dataset Information

Identification and Characterization of the Dermal Panniculus Carnosus Muscle Stem Cells.

ABSTRACT: The dermal Panniculus carnosus (PC) muscle is important for wound contraction in lower mammals and represents an interesting model of muscle regeneration due to its high cell turnover. The resident satellite cells (the bona fide muscle stem cells) remain poorly characterized. Here we analyzed PC satellite cells with regard to developmental origin and purported function. Lineage tracing shows that they originate in Myf5(+), Pax3/Pax7(+) cell populations. Skin and muscle wounding increased PC myofiber turnover, with the satellite cell progeny being involved in muscle regeneration but with no detectable contribution to the wound-bed myofibroblasts. Since hematopoietic stem cells fuse to PC myofibers in the absence of injury, we also studied the contribution of bone marrow-derived cells to the PC satellite cell compartment, demonstrating that cells of donor origin are capable of repopulating the PC muscle stem cell niche after irradiation and bone marrow transplantation but may not fully acquire the relevant myogenic commitment.

SUBMITTER: Naldaiz-Gastesi N

PROVIDER: S-EPMC5032673 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature

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Publications

Identification and Characterization of the Dermal Panniculus Carnosus Muscle Stem Cells.

Naldaiz-Gastesi Neia N Goicoechea María M Alonso-Martín Sonia S Aiastui Ana A López-Mayorga Macarena M García-Belda Paula P Lacalle Jaione J San José Carlos C Araúzo-Bravo Marcos J MJ Trouilh Lidwine L Anton-Leberre Véronique V Herrero Diego D Matheu Ander A Bernad Antonio A García-Verdugo José Manuel JM Carvajal Jaime J JJ Relaix Frédéric F Lopez de Munain Adolfo A García-Parra Patricia P Izeta Ander A

Stem cell reports 20160901 3

The dermal Panniculus carnosus (PC) muscle is important for wound contraction in lower mammals and represents an interesting model of muscle regeneration due to its high cell turnover. The resident satellite cells (the bona fide muscle stem cells) remain poorly characterized. Here we analyzed PC satellite cells with regard to developmental origin and purported function. Lineage tracing shows that they originate in Myf5(+), Pax3/Pax7(+) cell populations. Skin and muscle wounding increased PC myof ...[more]

PMID: 27594590

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Project description:Human multipotent skin derived precursor cells (SKPs) are traditionally sourced from dissociated dermal tissues; therefore, donor availability may become limiting. Here we demonstrate that both normal and diseased adult human dermal fibroblasts (DF) pre-cultured in conventional monolayers are capable of forming SKPs (termed m-SKPs). Moreover, we show that these m-SKPs can be passaged and that cryopreservation of original fibroblast monolayer cultures does not reduce m-SKP yield; however, extensive monolayer passaging does. Like SKPs generated from dissociated dermis, these m-SKPs expressed nestin, fibronectin and versican at the protein level. At the transcriptional level, m-SKPs derived from normal adult human DF, expressed neural crest stem cell markers such as p75NTR, embryonic stem cell markers such as Nanog and the mesenchymal stem cell marker Dermo-1. Furthermore, appropriate stimuli induced m-SKPs to differentiate down either mesenchymal or neural lineages resulting in lipid accumulation, calcification and S100? or ?-III tubulin expression (with multiple processes). m-SKP yield was greater from neonatal foreskin cultures compared to those from adult DF cultures; however, the former showed a greater decrease in m-SKP forming capacity after extensive monolayer passaging. m-SKP yield was greater from adult DF cultures expressing more alpha-smooth muscle actin (?SMA). In turn, elevated ?SMA expression correlated with cells originating from specimens isolated from biopsies containing more terminal hair follicles; however, ?SMA expression was lost upon m-SKP formation. Others have shown that dissociated human hair follicle dermal papilla (DP) are a highly enriched source of SKPs. However, conversely and unexpectedly, monolayer cultured human hair follicle DP cells failed to form m-SKPs whereas those from the murine vibrissae follicles did. Collectively, these findings reveal the potential for using expanded DF cultures to produce SKPs, the heterogeneity of SKP forming potential of skin from distinct anatomical locations and ages, and question the progenitor status of human hair follicle DP cells.

Dataset Information

Identification and Characterization of the Dermal Panniculus Carnosus Muscle Stem Cells.

Publications

Identification and Characterization of the Dermal Panniculus Carnosus Muscle Stem Cells.

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