Bacterial Spheroplasts as a Model for Visualizing Membrane Translocation of Antimicrobial Peptides.
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ABSTRACT:
SUBMITTER: Wei L
PROVIDER: S-EPMC5038235 | biostudies-literature | 2016 Oct
REPOSITORIES: biostudies-literature
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Bacterial Spheroplasts as a Model for Visualizing Membrane Translocation of Antimicrobial Peptides.
Wei Lei L LaBouyer Maria A MA Darling Louise E O LE Elmore Donald E DE
Antimicrobial agents and chemotherapy 20160923 10
Studies attempting to characterize the membrane translocation of antimicrobial and cell-penetrating peptides are frequently limited by the resolution of conventional light microscopy. This study shows that spheroplasts provide a valuable approach to overcome these limits. Spheroplasts produce less ambiguous images and allow for more systematic analyses of localization. Data collected with spheroplasts are consistent with studies using normal bacterial cells and imply that a particular peptide ma ...[more]
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