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Re-engineering a NiFe hydrogenase to increase the H2 production bias while maintaining native levels of O2 tolerance.

ABSTRACT: Naturally occurring oxygen tolerant NiFe membrane bound hydrogenases have a conserved catalytic bias towards hydrogen oxidation which limits their technological value. We present an Escherichia coli Hyd-1 amino acid exchange that apparently causes the catalytic rate of H2 production to double but does not impact the O2 tolerance.

SUBMITTER: Flanagan LA 

PROVIDER: S-EPMC5040170 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature

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Re-engineering a NiFe hydrogenase to increase the H2 production bias while maintaining native levels of O2 tolerance.

Flanagan Lindsey A LA   Wright John J JJ   Roessler Maxie M MM   Moir James W JW   Parkin Alison A  

Chemical communications (Cambridge, England) 20160701 58

Naturally occurring oxygen tolerant NiFe membrane bound hydrogenases have a conserved catalytic bias towards hydrogen oxidation which limits their technological value. We present an Escherichia coli Hyd-1 amino acid exchange that apparently causes the catalytic rate of H2 production to double but does not impact the O2 tolerance. ...[more]

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