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ABSTRACT:
SUBMITTER: Hidalgo R
PROVIDER: S-EPMC4531817 | biostudies-literature | 2015 Jun
REPOSITORIES: biostudies-literature
Hidalgo Ricardo R Ash Philip A PA Healy Adam J AJ Vincent Kylie A KA
Angewandte Chemie (International ed. in English) 20150429 24
A novel in situ IR spectroscopic approach is demonstrated for the characterization of hydrogenase during catalytic turnover. E. coli hydrogenase 1 (Hyd-1) is adsorbed on a high surface-area carbon electrode and subjected to the same electrochemical control and efficient supply of substrate as in protein film electrochemistry during spectral acquisition. The spectra reveal that the active site state known as Ni-L, observed in other NiFe hydrogenases only under illumination or at cryogenic tempera ...[more]