Ontology highlight
ABSTRACT:
SUBMITTER: Gleghorn ML
PROVIDER: S-EPMC5041459 | biostudies-literature | 2016 Sep
REPOSITORIES: biostudies-literature
Gleghorn Michael L ML Zhao Jianbo J Turner Douglas H DH Maquat Lynne E LE
Nucleic acids research 20160610 17
We have solved at 1. 07: Å resolution the X-ray crystal structure of a polyriboadenylic acid (poly(rA)) parallel and continuous double helix. Fifty-nine years ago, double helices of poly(rA) were first proposed to form at acidic pH. Here, we show that 7-mer oligo(rA), i.e. rA7, hybridizes and overlaps in all registers at pH 3.5 to form stacked double helices that span the crystal. Under these conditions, rA7 forms well-ordered crystals, whereas rA6 forms fragile crystalline-like structures, and ...[more]