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DSBCapture: in situ capture and sequencing of DNA breaks.

ABSTRACT: Double-strand DNA breaks (DSBs) continuously arise and cause mutations and chromosomal rearrangements. Here, we present DSBCapture, a sequencing-based method that captures DSBs in situ and directly maps these at single-nucleotide resolution, enabling the study of DSB origin. DSBCapture shows substantially increased sensitivity and data yield compared with other methods. Using DSBCapture, we uncovered a striking relationship between DSBs and elevated transcription within nucleosome-depleted chromatin.

SUBMITTER: Lensing SV

PROVIDER: S-EPMC5045719 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature

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DSBCapture: in situ capture and sequencing of DNA breaks.

Lensing Stefanie V SV Marsico Giovanni G Hänsel-Hertsch Robert R Lam Enid Y EY Tannahill David D Balasubramanian Shankar S

Nature methods 20160815 10

Double-strand DNA breaks (DSBs) continuously arise and cause mutations and chromosomal rearrangements. Here, we present DSBCapture, a sequencing-based method that captures DSBs in situ and directly maps these at single-nucleotide resolution, enabling the study of DSB origin. DSBCapture shows substantially increased sensitivity and data yield compared with other methods. Using DSBCapture, we uncovered a striking relationship between DSBs and elevated transcription within nucleosome-depleted chrom ...[more]

PMID: 27525976

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DSBCapture: in situ capture and direct sequencing of dsDNA breaks

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