Ontology highlight
ABSTRACT:
SUBMITTER: Lemak A
PROVIDER: S-EPMC5046226 | biostudies-literature | 2014 Dec
REPOSITORIES: biostudies-literature
Structure (London, England : 1993) 20141106 12
Multidomain proteins in which individual domains are connected by linkers often possess inherent interdomain flexibility that significantly complicates their structural characterization in solution using either nuclear magnetic resonance (NMR) spectroscopy or small-angle X-ray scattering (SAXS) alone. Here, we report a protocol for joint refinement of flexible multidomain protein structures against NMR distance and angular restraints, residual dipolar couplings, and SAXS data. The protocol is ba ...[more]