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Genetically encoded optical activation of DNA recombination in human cells.

ABSTRACT: We developed two tightly regulated, light-activated Cre recombinase enzymes through site-specific incorporation of two genetically-encoded photocaged amino acids in human cells. Excellent optical off to on switching of DNA recombination was achieved. Furthermore, we demonstrated precise spatial control of Cre recombinase through patterned illumination.

SUBMITTER: Luo J

PROVIDER: S-EPMC5048445 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature

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Genetically encoded optical activation of DNA recombination in human cells.

Luo J J Arbely E E Zhang J J Chou C C Uprety R R Chin J W JW Deiters A A

Chemical communications (Cambridge, England) 20160601 55

We developed two tightly regulated, light-activated Cre recombinase enzymes through site-specific incorporation of two genetically-encoded photocaged amino acids in human cells. Excellent optical off to on switching of DNA recombination was achieved. Furthermore, we demonstrated precise spatial control of Cre recombinase through patterned illumination. ...[more]

PMID: 27277957

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