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Genetically Encoded Protein Tyrosine Nitration in Mammalian Cells.

ABSTRACT: Tyrosine nitration has served as a major biomarker for oxidative stress and is present in high abundance in over 50 disease pathologies in humans. While data mounts on specific disease pathways from specific sites of tyrosine nitration, the role of these modifications is still largely unclear. Strategies for installing site-specific tyrosine nitration in target proteins in eukaryotic cells, through routes not dependent on oxidative stress, would provide a powerful method to address the consequences of tyrosine nitration. Developed here is a Methanosarcina barkeri aminoacyl-tRNA synthetase/tRNA pair that efficiently incorporates nitrotyrosine site-specifically into proteins in mammalian cells. We demonstrate the utility of this approach to produce nitrated proteins identified in disease conditions by producing site-specific nitroTyr-containing manganese superoxide dismutase and 14-3-3 proteins in eukaryotic cells.

SUBMITTER: Porter JJ

PROVIDER: S-EPMC7773054 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Genetically Encoded Protein Tyrosine Nitration in Mammalian Cells.

Porter Joseph J JJ Jang Hyo Sang HS Van Fossen Elise M EM Nguyen Duy P DP Willi Taylor S TS Cooley Richard B RB Mehl Ryan A RA

ACS chemical biology 20190604 6

Tyrosine nitration has served as a major biomarker for oxidative stress and is present in high abundance in over 50 disease pathologies in humans. While data mounts on specific disease pathways from specific sites of tyrosine nitration, the role of these modifications is still largely unclear. Strategies for installing site-specific tyrosine nitration in target proteins in eukaryotic cells, through routes not dependent on oxidative stress, would provide a powerful method to address the consequen ...[more]

PMID: 31117397

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