Ontology highlight
ABSTRACT:
SUBMITTER: Sendy B
PROVIDER: S-EPMC5052750 | biostudies-literature | 2016 Nov
REPOSITORIES: biostudies-literature
Philosophical transactions of the Royal Society of London. Series B, Biological sciences 20161101 1707
Chromatin immunoprecipitation, followed by quantification of immunoprecipitated DNA, can be used to measure RNA polymerase binding to any DNA segment in Escherichia coli By calibrating measurements against the signal from a single RNA polymerase bound at a single promoter, we can calculate both promoter occupancy levels and the flux of transcribing RNA polymerase through transcription units. Here, we have applied the methodology to the E. coli lactose operon promoter. We confirm that promoter oc ...[more]