Ontology highlight
ABSTRACT:
SUBMITTER: Martello R
PROVIDER: S-EPMC5056437 | biostudies-literature | 2016 Sep
REPOSITORIES: biostudies-literature
Martello Rita R Leutert Mario M Jungmichel Stephanie S Bilan Vera V Larsen Sara C SC Young Clifford C Hottiger Michael O MO Nielsen Michael L ML
Nature communications 20160930
Although protein ADP-ribosylation is involved in diverse biological processes, it has remained a challenge to identify ADP-ribose acceptor sites. Here, we present an experimental workflow for sensitive and unbiased analysis of endogenous ADP-ribosylation sites, capable of detecting more than 900 modification sites in mammalian cells and mouse liver. In cells, we demonstrate that Lys residues, besides Glu, Asp and Arg residues, are the dominant in vivo targets of ADP-ribosylation during oxidative ...[more]