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Lipocalin 2 prevents intestinal inflammation by enhancing phagocytic bacterial clearance in macrophages.

ABSTRACT: Lipocalin 2 (Lcn2), also called neutrophil gelatinase B-associated lipocalin (NGAL), is an anti-microbial peptide originally identified in neutrophil granules. Although Lcn2/NGAL expression is increased in the inflamed intestinal tissues of patients with inflammatory bowel disease, the role of Lcn2/NGAL in the development of intestinal inflammation remains unclear. Here we investigated the role of Lcn2/NGAL in intestinal inflammation using a spontaneous mouse colitis model, interleukin-10 knock out (IL-10 KO) mice. Lcn2 expression in the colonic tissues of IL-10 KO mice increased with the development of colitis. Lcn2/IL-10 double-KO mice showed a more rapid onset and development of colitis compared to IL-10 KO mice. Lcn2 enhanced phagocytic bacterial clearance in macrophages in vitro after infection with Escherichia coli. Transfer of Lcn2-repleted macrophages prevented the development of colitis in Lcn2/IL-10 double-KO mice in vivo. Our findings revealed that Lcn2 prevents the development of intestinal inflammation. One crucial factor seems to be the enhancement of phagocytic bacterial clearance in macrophages by Lcn2.

SUBMITTER: Toyonaga T

PROVIDER: S-EPMC5062163 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature

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Lipocalin 2 prevents intestinal inflammation by enhancing phagocytic bacterial clearance in macrophages.

Toyonaga Takahiko T Matsuura Minoru M Mori Kiyoshi K Honzawa Yusuke Y Minami Naoki N Yamada Satoshi S Kobayashi Taku T Hibi Toshifumi T Nakase Hiroshi H

Scientific reports 20161013

Lipocalin 2 (Lcn2), also called neutrophil gelatinase B-associated lipocalin (NGAL), is an anti-microbial peptide originally identified in neutrophil granules. Although Lcn2/NGAL expression is increased in the inflamed intestinal tissues of patients with inflammatory bowel disease, the role of Lcn2/NGAL in the development of intestinal inflammation remains unclear. Here we investigated the role of Lcn2/NGAL in intestinal inflammation using a spontaneous mouse colitis model, interleukin-10 knock ...[more]

PMID: 27734904

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Lipocalin 2 prevents intestinal inflammation by enhancing phagocytic bacterial clearance in macrophages.

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Lipocalin 2 prevents intestinal inflammation by enhancing phagocytic bacterial clearance in macrophages.

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