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Line-scanning Brillouin microscopy for rapid non-invasive mechanical imaging.

ABSTRACT: Brillouin spectroscopy probes the mechanical properties of material by measuring the optical frequency shift induced by photon-phonon scattering interactions. In traditional configurations, Brillouin spectrometers measure only one point of the sample at a time. This results in long acquisition times for mechanical imaging of large areas. In this work, we demonstrate a parallel detection configuration where the Brillouin shift of hundreds of points in a line can be measured simultaneously. In mm-sized samples, this novel configuration effectively shortens the acquisition time of two-dimensional Brillouin imaging from hours to tens of seconds, thus making it a powerful technology for label-free mechanical characterization of tissue and biomaterials.

SUBMITTER: Zhang J

PROVIDER: S-EPMC5064313 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature

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Line-scanning Brillouin microscopy for rapid non-invasive mechanical imaging.

Zhang Jitao J Fiore Antonio A Yun Seok-Hyun SH Kim Hanyoup H Scarcelli Giuliano G

Scientific reports 20161014

Brillouin spectroscopy probes the mechanical properties of material by measuring the optical frequency shift induced by photon-phonon scattering interactions. In traditional configurations, Brillouin spectrometers measure only one point of the sample at a time. This results in long acquisition times for mechanical imaging of large areas. In this work, we demonstrate a parallel detection configuration where the Brillouin shift of hundreds of points in a line can be measured simultaneously. In mm- ...[more]

PMID: 27739499

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