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Biomechanics of subcellular structures by non-invasive Brillouin microscopy.

ABSTRACT: Cellular biomechanics play a pivotal role in the pathophysiology of several diseases. Unfortunately, current methods to measure biomechanical properties are invasive and mostly limited to the surface of a cell. As a result, the mechanical behaviour of subcellular structures and organelles remains poorly characterised. Here, we show three-dimensional biomechanical images of single cells obtained with non-invasive, non-destructive Brillouin microscopy with an unprecedented spatial resolution. Our results quantify the longitudinal elastic modulus of subcellular structures. In particular, we found the nucleoli to be stiffer than both the nuclear envelope (p?

SUBMITTER: Antonacci G

PROVIDER: S-EPMC5109042 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature

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Biomechanics of subcellular structures by non-invasive Brillouin microscopy.

Antonacci Giuseppe G Braakman Sietse S

Scientific reports 20161115

Cellular biomechanics play a pivotal role in the pathophysiology of several diseases. Unfortunately, current methods to measure biomechanical properties are invasive and mostly limited to the surface of a cell. As a result, the mechanical behaviour of subcellular structures and organelles remains poorly characterised. Here, we show three-dimensional biomechanical images of single cells obtained with non-invasive, non-destructive Brillouin microscopy with an unprecedented spatial resolution. Our ...[more]

PMID: 27845411

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