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Direct observation of redox reactions in Candida parapsilosis ATCC 7330 by Confocal microscopic studies.

ABSTRACT: Confocal microscopic studies with the resting cells of yeast, Candida parapsilosis ATCC 7330, a reportedly versatile biocatalyst for redox enzyme mediated preparation of optically pure secondary alcohols in high optical purities [enantiomeric excess (ee) up to >99%] and yields, revealed that the yeast cells had large vacuoles under the experimental conditions studied where the redox reaction takes place. A novel fluorescence method was developed using 1-(6-methoxynaphthalen-2-yl)ethanol to track the site of biotransformation within the cells. This alcohol, itself non-fluorescent, gets oxidized to produce a fluorescent ketone, 1-(6-methoxynaphthalen-2-yl)ethanone. Kinetic studies showed that the reaction occurs spontaneously and the products get released out of the cells in less time [5?mins]. The biotransformation was validated using HPLC.

SUBMITTER: Venkataraman S

PROVIDER: S-EPMC5064409 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature

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Direct observation of redox reactions in Candida parapsilosis ATCC 7330 by Confocal microscopic studies.

Venkataraman Sowmyalakshmi S Narayan Shoba S Chadha Anju A

Scientific reports 20161014

Confocal microscopic studies with the resting cells of yeast, Candida parapsilosis ATCC 7330, a reportedly versatile biocatalyst for redox enzyme mediated preparation of optically pure secondary alcohols in high optical purities [enantiomeric excess (ee) up to >99%] and yields, revealed that the yeast cells had large vacuoles under the experimental conditions studied where the redox reaction takes place. A novel fluorescence method was developed using 1-(6-methoxynaphthalen-2-yl)ethanol to track ...[more]

PMID: 27739423

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