Dataset Information

Monolignol ferulate conjugates are naturally incorporated into plant lignins.

ABSTRACT: Angiosperms represent most of the terrestrial plants and are the primary research focus for the conversion of biomass to liquid fuels and coproducts. Lignin limits our access to fibers and represents a large fraction of the chemical energy stored in plant cell walls. Recently, the incorporation of monolignol ferulates into lignin polymers was accomplished via the engineering of an exotic transferase into commercially relevant poplar. We report that various angiosperm species might have convergently evolved to natively produce lignins that incorporate monolignol ferulate conjugates. We show that this activity may be accomplished by a BAHD feruloyl-coenzyme A monolignol transferase, OsFMT1 (AT5), in rice and its orthologs in other monocots.

SUBMITTER: Karlen SD

PROVIDER: S-EPMC5065250 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature

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Monolignol ferulate conjugates are naturally incorporated into plant lignins.

Karlen Steven D SD Zhang Chengcheng C Peck Matthew L ML Smith Rebecca A RA Padmakshan Dharshana D Helmich Kate E KE Free Heather C A HC Lee Seonghee S Smith Bronwen G BG Lu Fachuang F Sedbrook John C JC Sibout Richard R Grabber John H JH Runge Troy M TM Mysore Kirankumar S KS Harris Philip J PJ Bartley Laura E LE Ralph John J

Science advances 20161014 10

Angiosperms represent most of the terrestrial plants and are the primary research focus for the conversion of biomass to liquid fuels and coproducts. Lignin limits our access to fibers and represents a large fraction of the chemical energy stored in plant cell walls. Recently, the incorporation of monolignol ferulates into lignin polymers was accomplished via the engineering of an exotic transferase into commercially relevant poplar. We report that various angiosperm species might have convergen ...[more]

PMID: 27757415

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Project description:A new chemical modification protocol to generate N-lignins is presented, based on Indulin AT and Mg2+-lignosulfonate. The already known ammonoxidation reaction in liquid phase was used as a starting point and stepwise optimised towards a full solid-state approach. The "classical" liquid ammonoxidation products, the transition products from the optimization trials, as well as the "solid-state" products were comprehensively analysed and compared to the literature. The N-lignins obtained with the conventional ammonoxidation protocol showed the same properties as reported. Their molar mass distributions and the hydroxy group contents, hitherto not accessible due to solubility problems, were measured according to a recently reported protocol. N-Indulin showed an N-content up to 11 wt% and N-lignosulfonate up to 16 wt%. The transition experiments from liquid to solid-state gave insights into the influence of chemical components and reaction conditions. The use of a single chemical, the urea-hydrogen peroxide complex (UHP, "carbamide peroxide"), was sufficient to generate N-lignins with satisfying N-content. This chemical acts both as an N-source and as the oxidant. Following the optimization, a series of solid-state ammonoxidation tests were carried out. High N-contents of 10% in the case of Indulin and 11% in the case of lignosulfonate were obtained. By varying the ratio of UHP to lignin, the N-content can be controlled. Structural analysis showed that the N is organically bound to the lignin, similar to the "classical" ammonoxidation products obtained under homogeneous conditions. Overall, a new ammonoxidation protocol was developed which does not require an external gas supply nor liquids or dissolved reactants. This opens the possibility for carrying out the lignin modification in closed continuous reactor systems, such as extruders. The new, facile solid-state protocol will hopefully help N-lignins to find more consideration as a fertilizing material and in soil-improving materials.

Dataset Information

Monolignol ferulate conjugates are naturally incorporated into plant lignins.

Publications

Monolignol ferulate conjugates are naturally incorporated into plant lignins.

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