Ontology highlight
ABSTRACT:
SUBMITTER: Otsuka S
PROVIDER: S-EPMC5065316 | biostudies-literature | 2016 Sep
REPOSITORIES: biostudies-literature
Otsuka Shotaro S Bui Khanh Huy KH Schorb Martin M Hossain M Julius MJ Politi Antonio Z AZ Koch Birgit B Eltsov Mikhail M Beck Martin M Ellenberg Jan J
eLife 20160915
The nuclear pore complex (NPC) mediates nucleocytoplasmic transport through the nuclear envelope. How the NPC assembles into this double membrane boundary has remained enigmatic. Here, we captured temporally staged assembly intermediates by correlating live cell imaging with high-resolution electron tomography and super-resolution microscopy. Intermediates were dome-shaped evaginations of the inner nuclear membrane (INM), that grew in diameter and depth until they fused with the flat outer nucle ...[more]