Dataset Information

Precise Somatotopic Thalamocortical Axon Guidance Depends on LPA-Mediated PRG-2/Radixin Signaling.

ABSTRACT: Precise connection of thalamic barreloids with their corresponding cortical barrels is critical for processing of vibrissal sensory information. Here, we show that PRG-2, a phospholipid-interacting molecule, is important for thalamocortical axon guidance. Developing thalamocortical fibers both in PRG-2 full knockout (KO) and in thalamus-specific KO mice prematurely entered the cortical plate, eventually innervating non-corresponding barrels. This misrouting relied on lost axonal sensitivity toward lysophosphatidic acid (LPA), which failed to repel PRG-2-deficient thalamocortical fibers. PRG-2 electroporation in the PRG-2^-/- thalamus restored the aberrant cortical innervation. We identified radixin as a PRG-2 interaction partner and showed that radixin accumulation in growth cones and its LPA-dependent phosphorylation depend on its binding to specific regions within the C-terminal region of PRG-2. In vivo recordings and whisker-specific behavioral tests demonstrated sensory discrimination deficits in PRG-2^-/- animals. Our data show that bioactive phospholipids and PRG-2 are critical for guiding thalamic axons to their proper cortical targets.

SUBMITTER: Cheng J

PROVIDER: S-EPMC5065528 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature

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Precise Somatotopic Thalamocortical Axon Guidance Depends on LPA-Mediated PRG-2/Radixin Signaling.

Cheng Jin J Sahani Sadhna S Hausrat Torben Johann TJ Yang Jenq-Wei JW Ji Haichao H Schmarowski Nikolai N Endle Heiko H Liu Xinfeng X Li Yunbo Y Böttche Rahel R Radyushkin Konstantin K Maric Hans M HM Hoerder-Suabedissen Anna A Molnár Zoltán Z Prouvot Pierre-Hugues PH Trimbuch Thorsten T Ninnemann Olaf O Huai Jisen J Fan Wei W Visentin Barbara B Sabbadini Roger R Strømgaard Kristian K Stroh Albrecht A Luhmann Heiko J HJ Kneussel Matthias M Nitsch Robert R Vogt Johannes J

Neuron 20160915 1

Precise connection of thalamic barreloids with their corresponding cortical barrels is critical for processing of vibrissal sensory information. Here, we show that PRG-2, a phospholipid-interacting molecule, is important for thalamocortical axon guidance. Developing thalamocortical fibers both in PRG-2 full knockout (KO) and in thalamus-specific KO mice prematurely entered the cortical plate, eventually innervating non-corresponding barrels. This misrouting relied on lost axonal sensitivity towa ...[more]

PMID: 27641493

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Dataset Information

Precise Somatotopic Thalamocortical Axon Guidance Depends on LPA-Mediated PRG-2/Radixin Signaling.

Publications

Precise Somatotopic Thalamocortical Axon Guidance Depends on LPA-Mediated PRG-2/Radixin Signaling.

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