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Condensin I-mediated mitotic chromosome assembly requires association with chromokinesin KIF4A.

ABSTRACT: The chromokinesin KIF4A has been implicated in shaping mitotic chromosomes, but its functional relationship to condensin complexes remains controversial. Here, we found that, in mitosis, KIF4A associates with condensin I but not with condensin II. Mutational analyses indicated that the enrichment of condensin I to chromosomal axes depends on its association with KIF4A in a way that likely involves its motor activity. Remarkably, this interaction is required for condensin I to confer physiological properties to chromosomes. These observations provide an insight into how condensin I is enriched at chromosomal axes and underscore the significance of axial structure in organizing mitotic chromosomes.

SUBMITTER: Takahashi M

PROVIDER: S-EPMC5066236 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature

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Condensin I-mediated mitotic chromosome assembly requires association with chromokinesin KIF4A.

Takahashi Motoko M Wakai Toshifumi T Hirota Toru T

Genes & development 20160915 17

The chromokinesin KIF4A has been implicated in shaping mitotic chromosomes, but its functional relationship to condensin complexes remains controversial. Here, we found that, in mitosis, KIF4A associates with condensin I but not with condensin II. Mutational analyses indicated that the enrichment of condensin I to chromosomal axes depends on its association with KIF4A in a way that likely involves its motor activity. Remarkably, this interaction is required for condensin I to confer physiologica ...[more]

PMID: 27633014

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