Condensin controls mitotic chromosome stiffness and stability without forming a structurally contiguous scaffold.

ABSTRACT: During cell division, chromosomes must be folded into their compact mitotic form to ensure their segregation. This process is thought to be largely controlled by the action of condensin SMC protein complexes on chromatin fibers. However, how condensins organize metaphase chromosomes is not understood. We have combined micromanipulation of single human mitotic chromosomes, sub-nanonewton force measurement, siRNA interference of condensin subunit expression, and fluorescence microscopy, to analyze the role of condensin in large-scale chromosome organization. Condensin depletion leads to a dramatic (~ 10-fold) reduction in chromosome elastic stiffness relative to the native, non-depleted case. We also find that prolonged metaphase stalling of cells leads to overloading of chromosomes with con

SUBMITTER: Sun M 

PROVIDER: S-EPMC6370136 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature