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Complement pathway amplifies caspase-11-dependent cell death and endotoxin-induced sepsis severity.

ABSTRACT: Cell death and release of proinflammatory mediators contribute to mortality during sepsis. Specifically, caspase-11-dependent cell death contributes to pathology and decreases in survival time in sepsis models. Priming of the host cell, through TLR4 and interferon receptors, induces caspase-11 expression, and cytosolic LPS directly stimulates caspase-11 activation, promoting the release of proinflammatory cytokines through pyroptosis and caspase-1 activation. Using a CRISPR-Cas9-mediated genome-wide screen, we identified novel mediators of caspase-11-dependent cell death. We found a complement-related peptidase, carboxypeptidase B1 (Cpb1), to be required for caspase-11 gene expression and subsequent caspase-11-dependent cell death. Cpb1 modifies a cleavage product of C3, which binds to and activates C3aR, and then modulates innate immune signaling. We find the Cpb1-C3-C3aR pathway induces caspase-11 expression through amplification of MAPK activity downstream of TLR4 and Ifnar activation, and mediates severity of LPS-induced sepsis (endotoxemia) and disease outcome in mice. We show C3aR is required for up-regulation of caspase-11 orthologues, caspase-4 and -5, in primary human macrophages during inflammation and that c3aR1 and caspase-5 transcripts are highly expressed in patients with severe sepsis; thus, suggesting that these pathways are important in human sepsis. Our results highlight a novel role for complement and the Cpb1-C3-C3aR pathway in proinflammatory signaling, caspase-11 cell death, and sepsis severity.

SUBMITTER: Napier BA

PROVIDER: S-EPMC5068231 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature

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Complement pathway amplifies caspase-11-dependent cell death and endotoxin-induced sepsis severity.

Napier Brooke A BA Brubaker Sky W SW Sweeney Timothy E TE Monette Patrick P Rothmeier Greggory H GH Gertsvolf Nina A NA Puschnik Andreas A Carette Jan E JE Khatri Purvesh P Monack Denise M DM

The Journal of experimental medicine 20161003 11

Cell death and release of proinflammatory mediators contribute to mortality during sepsis. Specifically, caspase-11-dependent cell death contributes to pathology and decreases in survival time in sepsis models. Priming of the host cell, through TLR4 and interferon receptors, induces caspase-11 expression, and cytosolic LPS directly stimulates caspase-11 activation, promoting the release of proinflammatory cytokines through pyroptosis and caspase-1 activation. Using a CRISPR-Cas9-mediated genome- ...[more]

PMID: 27697835

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Complement pathway amplifies caspase-11-dependent cell death and endotoxin-induced sepsis severity.

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Complement pathway amplifies caspase-11-dependent cell death and endotoxin-induced sepsis severity.

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