Ontology highlight
ABSTRACT:
SUBMITTER: Serdiuk T
PROVIDER: S-EPMC5069129 | biostudies-literature | 2016 Nov
REPOSITORIES: biostudies-literature
Serdiuk Tetiana T Balasubramaniam Dhandayuthapani D Sugihara Junichi J Mari Stefania A SA Kaback H Ronald HR Müller Daniel J DJ
Nature chemical biology 20160905 11
How chaperones, insertases and translocases facilitate insertion and folding of complex cytoplasmic proteins into cellular membranes is not fully understood. Here we utilize single-molecule force spectroscopy to observe YidC, a transmembrane chaperone and insertase, sculpting the folding trajectory of the polytopic α-helical membrane protein lactose permease (LacY). In the absence of YidC, unfolded LacY inserts individual structural segments into the membrane; however, misfolding dominates the p ...[more]