Rapid Exchange Between Free and Bound States in RNA-Dendrimer Polyplexes: Implications on the Mechanism of Delivery and Release.
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ABSTRACT:
SUBMITTER: Shakya A
PROVIDER: S-EPMC5070374 | biostudies-literature | 2016 Jan
REPOSITORIES: biostudies-literature
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Rapid Exchange Between Free and Bound States in RNA-Dendrimer Polyplexes: Implications on the Mechanism of Delivery and Release.
Shakya Anisha A Dougherty Casey A CA Xue Yi Y Al-Hashimi Hashim M HM Banaszak Holl Mark M MM
Biomacromolecules 20151211 1
A combination of solution NMR, dynamic light scattering (DLS), and fluorescence quenching assays were employed to obtain insights into the dynamics and structural features of a polyplex system consisting of HIV-1 transactivation response element (TAR) and PEGylated generation 5 poly(amidoamine) dendrimer (G5-PEG). NMR chemical shift mapping and (13)C spin relaxation based dynamics measurements depict the polyplex system as a highly dynamic assembly where the RNA, with its local structure and dyn ...[more]
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