Ontology highlight
ABSTRACT:
SUBMITTER: Venkataram S
PROVIDER: S-EPMC5070919 | biostudies-literature | 2016 Sep
REPOSITORIES: biostudies-literature
Venkataram Sandeep S Dunn Barbara B Li Yuping Y Agarwala Atish A Chang Jessica J Ebel Emily R ER Geiler-Samerotte Kerry K Hérissant Lucas L Blundell Jamie R JR Levy Sasha F SF Fisher Daniel S DS Sherlock Gavin G Petrov Dmitri A DA
Cell 20160901 6
Adaptive evolution plays a large role in generating the phenotypic diversity observed in nature, yet current methods are impractical for characterizing the molecular basis and fitness effects of large numbers of individual adaptive mutations. Here, we used a DNA barcoding approach to generate the genotype-to-fitness map for adaptation-driving mutations from a Saccharomyces cerevisiae population experimentally evolved by serial transfer under limiting glucose. We isolated and measured the fitness ...[more]