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Symmetry-mismatch reconstruction of genomes and associated proteins within icosahedral viruses using cryo-EM.

ABSTRACT: Although near-atomic resolutions have been routinely achieved for structural determination of many icosahedral viral capsids, structures of genomes and associated proteins within the capsids are still less characterized because the genome information is overlapped by the highly symmetric capsid information in the virus particle images. We recently developed a software package for symmetry-mismatch structural reconstruction and determined the structures of the genome and RNA polymerases within an icosahedral virus for the first time. Here, we describe the protocol used for this structural determination, which may facilitate structural biologists in investigating the structures of viral genome and associated proteins.

SUBMITTER: Li X

PROVIDER: S-EPMC5071370 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Symmetry-mismatch reconstruction of genomes and associated proteins within icosahedral viruses using cryo-EM.

Li Xiaowu X Liu Hongrong H Cheng Lingpeng L

Biophysics reports 20160527 1

Although near-atomic resolutions have been routinely achieved for structural determination of many icosahedral viral capsids, structures of genomes and associated proteins within the capsids are still less characterized because the genome information is overlapped by the highly symmetric capsid information in the virus particle images. We recently developed a software package for symmetry-mismatch structural reconstruction and determined the structures of the genome and RNA polymerases within an ...[more]

PMID: 27819028

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