Ontology highlight
ABSTRACT:
SUBMITTER: Barthel AC
PROVIDER: S-EPMC3903049 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Barthel Andrew C AC Tagare Hemant H Sigworth Fred J FJ
Conference record. Asilomar Conference on Signals, Systems & Computers 20110101
Random spherically-constrained (RSC) reconstruction is a new form of single particle reconstruction (SPR) using cryo-EM images of membrane proteins embedded in spherical lipid vesicles to generate a 3D protein structure. The method has many advantages over conventional SPR, including a more native environment for protein particles and an initial estimate of the particle's angular orientation. These advances allow us to determine structures of membrane proteins such as ion channels and derive mor ...[more]