Ontology highlight
ABSTRACT:
SUBMITTER: Krinsky N
PROVIDER: S-EPMC5074584 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
PloS one 20161021 10
Cell-free protein synthesis (CFPS) systems are important laboratory tools that are used for various synthetic biology applications. Here, we present a simple and inexpensive laboratory-scale method for preparing a CFPS system from E. coli. The procedure uses basic lab equipment, a minimal set of reagents, and requires less than one hour to process the bacterial cell mass into a functional S30-T7 extract. BL21(DE3) and MRE600 E. coli strains were used to prepare the S30-T7 extract. The CFPS syste ...[more]