Ontology highlight
ABSTRACT:
SUBMITTER: Yates JD
PROVIDER: S-EPMC8682767 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature
Yates Joshua D JD Russell Robert C RC Barton Nathaniel J NJ Yost H Joseph HJ Hill Jonathon T JT
Nucleic acids research 20211201 22
CRISPR-Cas9 sgRNA libraries have transformed functional genetic screening and have enabled several innovative methods that rely on simultaneously targeting numerous genetic loci. Such libraries could be used in a vast number of biological systems and in the development of new technologies, but library generation is hindered by the cost, time, and sequence data required for sgRNA library synthesis. Here, we describe a rapid enzymatic method for generating robust, variant-matched libraries from an ...[more]