Ontology highlight
ABSTRACT:
SUBMITTER: Zhong W
PROVIDER: S-EPMC5080546 | biostudies-literature | 2016 Oct
REPOSITORIES: biostudies-literature
Zhong Weilong W Sun Bo B Lu Cheng C Yu Hengheng H Wang Changhua C He Lingfei L Gu Ju J Chen Shuang S Liu Yanrong Y Jing Xiangyan X Bi Zhun Z Yang Guang G Zhou Honggang H Sun Tao T Yang Cheng C
Scientific reports 20161026
Small-molecule fluorescent probes have been widely used in target identification, but this method has many disadvantages. For example, the identified proteins are usually complex, and additional biochemical studies are needed to distinguish real targets from interference results. To address this problem, we propose a series of strategies for improving the efficiency of target identification. First, pretreatment with a lower concentration of hydrogen peroxide can shield against thiol interference ...[more]