Dataset Information

Depot-specific differences in angiogenic capacity of adipose tissue in differential susceptibility to diet-induced obesity.

ABSTRACT:

Objective

Adipose tissue (AT) expansion requires AT remodeling, which depends on AT angiogenesis. Modulation of AT angiogenesis could have therapeutic promise for the treatment of obesity. However, it is unclear how the capacity of angiogenesis in each adipose depot is affected by over-nutrition. Therefore, we investigated the angiogenic capacity (AC) of subcutaneous and visceral fats in lean and obese mice.

Methods

We compared the AC of epididymal fat (EF) and inguinal fat (IF) using an angiogenesis assay in diet-induced obese (DIO) mice and diet-resistant (DR) mice fed a high-fat diet (HFD). Furthermore, we compared the expression levels of genes related to angiogenesis, macrophage recruitment, and inflammation using RT-qPCR in the EF and IF of lean mice fed a low-fat diet (LFD), DIO mice, and DR mice fed a HFD.

Results

DIO mice showed a significant increase in the AC of EF only at 22 weeks of age compared to DR mice. The expression levels of genes related to angiogenesis, macrophage recruitment, and inflammation were significantly higher in the EF of DIO mice than in those of LFD mice and DR mice, while expression levels of genes related to macrophages and their recruitment were higher in the IF of DIO mice than in those of LFD and DR mice. Expression of genes related to angiogenesis (including Hif1a, Vegfa, Fgf1, Kdr, and Pecam1), macrophage recruitment, and inflammation (including Emr1, Ccr2, Itgax, Ccl2, Tnf, and Il1b) correlated more strongly with body weight in the EF of HFD-fed obese mice compared to that of IF.

Conclusions

These results suggest depot-specific differences in AT angiogenesis and a potential role in the susceptibility to diet-induced obesity.

SUBMITTER: Song MG

PROVIDER: S-EPMC5081408 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature

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Publications

Depot-specific differences in angiogenic capacity of adipose tissue in differential susceptibility to diet-induced obesity.

Song Mun-Gyu MG Lee Hye-Jin HJ Jin Bo-Yeong BY Gutierrez-Aguilar Ruth R Shin Kyung-Ho KH Choi Sang-Hyun SH Um Sung Hee SH Kim Dong-Hoon DH

Molecular metabolism 20160909 11

<h4>Objective</h4>Adipose tissue (AT) expansion requires AT remodeling, which depends on AT angiogenesis. Modulation of AT angiogenesis could have therapeutic promise for the treatment of obesity. However, it is unclear how the capacity of angiogenesis in each adipose depot is affected by over-nutrition. Therefore, we investigated the angiogenic capacity (AC) of subcutaneous and visceral fats in lean and obese mice.<h4>Methods</h4>We compared the AC of epididymal fat (EF) and inguinal fat (IF) u ...[more]

PMID: 27818937

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Dataset Information

Depot-specific differences in angiogenic capacity of adipose tissue in differential susceptibility to diet-induced obesity.

Objective

Methods

Results

Conclusions

Publications

Depot-specific differences in angiogenic capacity of adipose tissue in differential susceptibility to diet-induced obesity.

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