Ontology highlight
ABSTRACT:
SUBMITTER: Hara S
PROVIDER: S-EPMC5081742 | biostudies-literature | 2016 Oct
REPOSITORIES: biostudies-literature
Hara Satoshi S Kato Tomoko T Goto Yuji Y Kubota Souichirou S Tamano Moe M Terao Miho M Takada Shuji S
The Journal of reproduction and development 20160708 5
The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system is a useful tool for genome editing. In this study, using a microinjection-based CRISPR/Cas9 system, we efficiently generated mouse lines carrying mutations at the Irx3 and Irx5 loci, which are located in close proximity on a chromosome and are functionally redundant. During the generation of Irx3/Irx5 double mutant mice, a deletion of ~0.5 Mb between the Irx3 and Irx5 loci was uninten ...[more]