4D Confocal Imaging of Yeast Organelles.
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ABSTRACT:
SUBMITTER: Day KJ
PROVIDER: S-EPMC5086802 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
Publications
4D Confocal Imaging of Yeast Organelles.
Day Kasey J KJ Papanikou Effrosyni E Glick Benjamin S BS
Methods in molecular biology (Clifton, N.J.) 20160101
Yeast cells are well suited to visualizing organelles by 4D confocal microscopy. Typically, one or more cellular compartments are labeled with a fluorescent protein or dye, and a stack of confocal sections spanning the entire cell volume is captured every few seconds. Under appropriate conditions, organelle dynamics can be observed for many minutes with only limited photobleaching. Images are captured at a relatively low signal-to-noise ratio and are subsequently processed to generate movies tha ...[more]
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