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Imaging the electrical activity of organelles in living cells.

ABSTRACT: Eukaryotic cells are complex systems compartmentalized in membrane-bound organelles. Visualization of organellar electrical activity in living cells requires both a suitable reporter and non-invasive imaging at high spatiotemporal resolution. Here we present hVoS_org, an optical method to monitor changes in the membrane potential of subcellular membranes. This method takes advantage of a FRET pair consisting of a membrane-bound voltage-insensitive fluorescent donor and a non-fluorescent voltage-dependent acceptor that rapidly moves across the membrane in response to changes in polarity. Compared to the currently available techniques, hVoS_org has advantages including simple and precise subcellular targeting, the ability to record from individual organelles, and the potential for optical multiplexing of organellar activity.

SUBMITTER: Matamala E

PROVIDER: S-EPMC7988155 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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Imaging the electrical activity of organelles in living cells.

Matamala Ella E Castillo Cristian C Vivar Juan P JP Rojas Patricio A PA Brauchi Sebastian E SE

Communications biology 20210323 1

Eukaryotic cells are complex systems compartmentalized in membrane-bound organelles. Visualization of organellar electrical activity in living cells requires both a suitable reporter and non-invasive imaging at high spatiotemporal resolution. Here we present hVoS<sub>org</sub>, an optical method to monitor changes in the membrane potential of subcellular membranes. This method takes advantage of a FRET pair consisting of a membrane-bound voltage-insensitive fluorescent donor and a non-fluorescen ...[more]

PMID: 33758369

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