Analysis of Chemotherapeutic Drug Delivery at the Single Cell Level Using 3D-MSI-TOF-SIMS.

ABSTRACT: In this work, we show the advantages of label-free, tridimensional mass spectrometry imaging using dual beam analysis (25 keV Bi₃⁺) and depth profiling (20 keV with a distribution centered at Ar₁₅₀₀⁺) coupled to time of flight secondary ion mass spectrometry (3D-MSI-TOF-SIMS) for the study of A-172 human glioblastoma cell line treated with B-cell lymphoma 2 (Bcl-2) inhibitor ABT-737. The high spatial (~250 nm) and high mass resolution (m/Δm ~10,000) of TOF-SIMS permitted the localization and identification of the intact, unlabeled drug molecular ion (m/z 811.26 C₄₂H₄₄ClN₆O₅S₂^- [M - H]^-) as well as characteristic fragment ions. We propose a novel approach based on the ins

SUBMITTER: Vanbellingen QP 

PROVIDER: S-EPMC5088064 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature