Unknown

Dataset Information

0

Derivation of Patient Specific Pluripotent Stem Cells Using Clinically Discarded Cumulus Cells.

ABSTRACT: Induced pluripotent stem cells (iPSCs) are powerful tools for basic and translational research, as well as regenerative medicine. In routine human in vitro fertilization (IVF) practices, cumulus cells (CCs) are discarded, representing a potential source of biological materials for regenerative medicine. In this study, we derived patient-specific iPSCs using CCs from human infertility clinics for the first time. The human cumulus cell derived iPSCs (hc-iPSCs) were characterized for growth, karyotype, expression of pluripotency genes, and were subjected to embryoid bodies (EBs) and teratoma assays to evaluate their differentiation capacity. Hc-iPSCs display typical iPSC characteristics, and are capable of differentiating into all germ layers in vitro and in vivo. We further show that putative primordial germ cell like cells (PGCLCs) can be derived using hc-iPSCs. Our data demonstrate the feasibility of deriving patient-specific pluripotent stem cells using CCs.

SUBMITTER: Chang WF 

PROVIDER: S-EPMC5089679 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Derivation of Patient Specific Pluripotent Stem Cells Using Clinically Discarded Cumulus Cells.

Chang Wei-Fang WF   Hwu Yuh-Ming YM   Xu Jie J   Lin Chen-Ju CJ   Wang Sheng-Wen SW   Cheng An-Sheng AS   Lu Jean J   Lu Chung-Hao CH   Sung Li-Ying LY  

PloS one 20161101 11

Induced pluripotent stem cells (iPSCs) are powerful tools for basic and translational research, as well as regenerative medicine. In routine human in vitro fertilization (IVF) practices, cumulus cells (CCs) are discarded, representing a potential source of biological materials for regenerative medicine. In this study, we derived patient-specific iPSCs using CCs from human infertility clinics for the first time. The human cumulus cell derived iPSCs (hc-iPSCs) were characterized for growth, karyot  ...[more]

Similar Datasets

Unknown Derivation and high engraftment of patient-specific cardiomyocyte sheet using induced pluripotent stem cells generated from adult cardiac fibroblast.
| S-EPMC4303534 | biostudies-literature
Unknown Cardiovascular Disease Modeling Using Patient-Specific Induced Pluripotent Stem Cells.
| S-EPMC4581278 | biostudies-literature
Unknown Modeling retinal degeneration using patient-specific induced pluripotent stem cells.
| S-EPMC3037398 | biostudies-literature
Unknown Modeling congenital cataract in vitro using patient-specific induced pluripotent stem cells.
| S-EPMC8486789 | biostudies-literature
Unknown Derivation of trophoblast stem cells from naive human pluripotent stem cells.
| S-EPMC7062471 | biostudies-literature
Unknown Derivation of Airway Basal Stem Cells from Human Pluripotent Stem Cells.
| S-EPMC7796997 | biostudies-literature
Unknown Derivation of autism spectrum disorder-specific induced pluripotent stem cells from peripheral blood mononuclear cells.
| S-EPMC4278654 | biostudies-literature
Unknown Derivation and functional analysis of patient-specific induced pluripotent stem cells as an in vitro model of chronic granulomatous disease.
| S-EPMC3593166 | biostudies-literature
Unknown Derivation of a disease-specific human induced pluripotent stem cell line from a biliary atresia patient.
| S-EPMC5881114 | biostudies-literature
Unknown A systematic evaluation of integration free reprogramming methods for deriving clinically relevant patient specific induced pluripotent stem (iPS) cells.
| S-EPMC3841145 | biostudies-literature