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A field-deployable mobile molecular diagnostic system for malaria at the point of need.

ABSTRACT: In response to the urgent need of a field-deployable and highly sensitive malaria diagnosis, we developed a standalone, "sample-in-answer-out" molecular diagnostic system (AnyMDx) to enable quantitative molecular analysis of blood-borne malaria in low resource areas. The system consists of a durable battery-powered analyzer and a disposable microfluidic compact disc loaded with reagents ready for use. A low power thermal module and a novel fluorescence-sensing module are integrated into the analyzer for real-time monitoring of loop-mediated isothermal nucleic acid amplification (LAMP) of target parasite DNA. With 10 ?L of raw blood sample, the AnyMDx system automates the nucleic acid sample preparation and subsequent LAMP and real-time detection. Under laboratory conditions with whole-blood samples spiked with cultured Plasmodium falciparum, we achieved a detection limit of ?0.6 parasite per ?L, much lower than those for the conventional microscopy and rapid diagnostic tests (?50-100 parasites per ?L). The turnaround time from sample to answer is less than 40 minutes. The AnyMDx is user-friendly requiring minimal technological training. The analyzer and the disposable reagent compact discs are cost-effective, making AnyMDx a potential tool for malaria molecular diagnosis under field settings for malaria elimination.

SUBMITTER: Choi G

PROVIDER: S-EPMC5089926 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature

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A field-deployable mobile molecular diagnostic system for malaria at the point of need.

Choi Gihoon G Song Daniel D Shrestha Sony S Miao Jun J Cui Liwang L Guan Weihua W

Lab on a chip 20161101 22

In response to the urgent need of a field-deployable and highly sensitive malaria diagnosis, we developed a standalone, "sample-in-answer-out" molecular diagnostic system (AnyMDx) to enable quantitative molecular analysis of blood-borne malaria in low resource areas. The system consists of a durable battery-powered analyzer and a disposable microfluidic compact disc loaded with reagents ready for use. A low power thermal module and a novel fluorescence-sensing module are integrated into the anal ...[more]

PMID: 27722377

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A field-deployable mobile molecular diagnostic system for malaria at the point of need.

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A field-deployable mobile molecular diagnostic system for malaria at the point of need.

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