Ontology highlight
ABSTRACT:
SUBMITTER: Wang J
PROVIDER: S-EPMC5090098 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
Wang Jing J Pan Xiaoming X Liang Xingguo X
Journal of analytical methods in chemistry 20161019
High resolution melting (HRM), with a high sensitivity to distinguish the nucleic acid species with small variations, has been widely applied in the mutation scanning, methylation analysis, and genotyping. For the aim of extending HRM for the evaluation of thermal stability of nucleic acid secondary structures on sequence dependence, we investigated effects of the dye of EvaGreen, metal ions, and impurities (such as dNTPs) on melting temperature (<i>T</i><sub><i>m</i></sub> ) measurement by HRM. ...[more]