Targeting HMGA2 in Retinoblastoma Cells in vitro Using the Aptamer Strategy.
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ABSTRACT:
SUBMITTER: Nalini V
PROVIDER: S-EPMC5091129 | biostudies-literature | 2016 Oct
REPOSITORIES: biostudies-literature
Publications
Targeting HMGA2 in Retinoblastoma Cells in vitro Using the Aptamer Strategy.
Nalini Venkatesan V Deepa Perinkulam Ravi PR Raguraman Rajeswari R Khetan Vikas V Reddy Maddy Ashwin MA Krishnakumar Subramanian S
Ocular oncology and pathology 20160702 4
High-mobility group A2 (HMGA2) protein regulates retinoblastoma (RB) cancer cell proliferation. Here, a stable phosphorothioate-modified HMGA2 aptamer was used to block HMGA2 protein function in RB cells. HMGA2-aptamer internalisation in RB cells (Y79, Weri Rb1) and non-neoplastic human retinal cells (MIO-M1) were optimised. Aptamer induced dose-dependent cytotoxicity in RB cancer cells (0.25-1.5 µM). Increased expression of <i>TGFβ</i>, <i>SMAD4</i>, <i>CDH1</i>, <i>BAX</i>, <i>CASP 3</i>, <i>P ...[more]
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