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Insights into Protein Structure and Dynamics by Ultraviolet and Visible Resonance Raman Spectroscopy.


ABSTRACT: Raman spectroscopy is a form of vibrational spectroscopy based on inelastic scattering of light. In resonance Raman spectroscopy, the wavelength of the incident light falls within an absorption band of a chromophore, and this overlap of excitation and absorption energy greatly enhances the Raman scattering efficiency of the absorbing species. The ability to probe vibrational spectra of select chromophores within a complex mixture of molecules makes resonance Raman spectroscopy an excellent tool for studies of biomolecules. In this Current Topic, we discuss the type of molecular insights obtained from steady-state and time-resolved resonance Raman studies of a prototypical photoactive protein, rhodopsin. We also review recent efforts in ultraviolet resonance Raman investigations of soluble and membrane-associated biomolecules, including integral membrane proteins and antimicrobial peptides. These examples illustrate that resonance Raman is a sensitive, selective, and practical method for studying the structures of biological molecules, and the molecular bonding, geometry, and environments of protein cofactors, the backbone, and side chains.

SUBMITTER: Lopez-Pena I 

PROVIDER: S-EPMC5092233 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

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Insights into Protein Structure and Dynamics by Ultraviolet and Visible Resonance Raman Spectroscopy.

López-Peña Ignacio I   Leigh Brian S BS   Schlamadinger Diana E DE   Kim Judy E JE  

Biochemistry 20150729 31


Raman spectroscopy is a form of vibrational spectroscopy based on inelastic scattering of light. In resonance Raman spectroscopy, the wavelength of the incident light falls within an absorption band of a chromophore, and this overlap of excitation and absorption energy greatly enhances the Raman scattering efficiency of the absorbing species. The ability to probe vibrational spectra of select chromophores within a complex mixture of molecules makes resonance Raman spectroscopy an excellent tool  ...[more]

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