Dataset Information

Reconstitution of Peripheral T Cells by Tissue-Derived CCR4+ Central Memory Cells Following HIV-1 Antiretroviral Therapy.

ABSTRACT:

Background

Highly active antiretroviral therapy induces clinical benefits to HIV-1 infected individuals, which can be striking in those with progressive disease. Improved survival and decreased incidence of opportunistic infections go hand in hand with a suppression of the plasma viral load, an increase in peripheral CD4⁺ T-cell counts, as well as a reduction in the activation status of both CD4⁺ and CD8⁺ T cells.

Methods

We investigated T-cell dynamics during ART by polychromatic flow cytometry in total as well as in HIV-1-specific CD4⁺ and CD8⁺ T cells in patients with advanced disease. We also measured gene expression by single cell transcriptomics to assess functional state.

Results

The cytokine pattern of HIV-specific CD8⁺ T cells was not altered after ART, though their magnitude decreased significantly as the plasma viral load was suppressed to undetectable levels. Importantly, while CD4⁺ T cell numbers increased substantially during the first year, the population did not normalize: the increases were largely due to expansion of mucosal-derived CCR4⁺ CD4⁺ T_CM; transcriptomic analysis revealed that these are not classical Th₂-type cells.

Conclusion

The apparent long-term normalization of CD4⁺ T-cell numbers following ART does not comprise a normal balance of functionally distinct cells, but results in a dramatic Th₂ shift of the reconstituting immune system.

SUBMITTER: Mahnke YD

PROVIDER: S-EPMC5093337 | biostudies-literature |

REPOSITORIES: biostudies-literature

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