Ontology highlight
ABSTRACT:
SUBMITTER: Nakanishi T
PROVIDER: S-EPMC5098252 | biostudies-literature | 2016 Nov
REPOSITORIES: biostudies-literature
Nakanishi Takashi T Kato Yasuhiko Y Matsuura Tomoaki T Watanabe Hajime H
Scientific reports 20161107
Transcription activator-like effector nucleases (TALENs) are versatile tools that enable the insertion of DNA into different organisms. Here, we confirmed TALEN-mediated knock-in via non-homologous end joining in the crustacean Daphnia magna, a model organism for ecological and toxicological genomics. We tested two different TALENs, ey1 TALEN and ey2 TALEN, both of which target the eyeless locus. The donor DNA plasmid, harbouring the H2B-GFP reporter gene, was designed to contain both TALEN targ ...[more]