Ontology highlight
ABSTRACT:
SUBMITTER: Sawatsubashi S
PROVIDER: S-EPMC5766535 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Sawatsubashi Shun S Joko Yudai Y Fukumoto Seiji S Matsumoto Toshio T Sugano Shigeo S SS
Scientific reports 20180112 1
CRISPR/Cas9-based genome editing has dramatically accelerated genome engineering. An important aspect of genome engineering is efficient knock-in technology. For improved knock-in efficiency, the non-homologous end joining (NHEJ) repair pathway has been used over the homology-dependent repair pathway, but there remains a need to reduce the complexity of the preparation of donor vectors. We developed the versatile NHEJ-based knock-in module for genome editing (VIKING). Using the consensus sequenc ...[more]