Ontology highlight
ABSTRACT:
SUBMITTER: Dorsett D
PROVIDER: S-EPMC5105155 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Dorsett Dale D Misulovin Ziva Z
Methods in molecular biology (Clifton, N.J.) 20170101
This chapter presents methods to conduct and analyze genome-wide chromatin immunoprecipitation of the cohesin complex and the Nipped-B cohesin loading factor in Drosophila cells using high-throughput DNA sequencing (ChIP-seq). Procedures for isolation of chromatin, immunoprecipitation, and construction of sequencing libraries for the Ion Torrent Proton high throughput sequencer are detailed, and computational methods to calculate occupancy as input-normalized fold-enrichment are described. The r ...[more]