Ontology highlight
ABSTRACT:
SUBMITTER: Chang HW
PROVIDER: S-EPMC5106197 | biostudies-literature | 2016 Nov
REPOSITORIES: biostudies-literature
Science advances 20161111 11
Efficient overcoming and accurate maintenance of chromatin structure and associated histone marks during DNA replication are essential for normal functioning of the daughter cells. However, the molecular mechanisms of replication through chromatin are unknown. We have studied traversal of uniquely positioned mononucleosomes by T7 replisome in vitro. Nucleosomes present a strong, sequence-dependent barrier for replication, with particularly strong pausing of DNA polymerase at the +(31-40) and +(4 ...[more]